Enzymology Lab Report Example | Topics and Well Written Essays - 1000 words

Enzymology - Lab Report Example Enzymes do accelerate reaction rate by more than 1 million g=folds, in that reactions would take a very long time to occur. Appropriate enzymes catalyze in few seconds and there activities, in many cases occur inside the cell, within the membranes of the cell. The rate of reaction when Ph, temperature, time and the substrate are at a constant is determined by the enzyme availability. Effects of enzymes in substrate concentration can be analysed as below. The concentration of enzymes is a factor in determining the rate of reaction. The substrate needs to be present in excess. That is to say that each reaction should be independent of the concentration of the substrate. Any possible change in product amount over a given time period depends upon present enzyme level. A and E reacting leads to B at a constant rate k1, this turns to C and generates E again at a constant rate k2. At that instance, B has the possibility of turning into A and E again at a constant rate k-1. Michaelis-Menten enzymatic catalysis mechanism follows this path. Competitive inhibition has a molecule similar to the present or available substrate but not able to be acted on by those enzymes that compete with them for sites that are active. Fewer active sites readily act on the substrate because of the inhibitor presence. Given the structure of the enzyme is not affected by the enzyme inhibitors; they will still act as catalysts for the reaction. In inhibition that is uncompetitive, molecules tend to bind to enzymes instead of sites that are active. That makes the three dimensional enzyme structures to change in that its site that is active still binds to the substrate in affinity that is usual, though not in the optimal arrangement of stabilizing the rate of transition and in turn catalyzing the reaction. Lineweaver Burk plots are significant in a number of ways. For instance, in the case where it is